Structural and functional analysis of the Francisella lysine decarboxylase as a key actor in oxidative stress resistance - Archive ouverte HAL
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Structural and functional analysis of the Francisella lysine decarboxylase as a key actor in oxidative stress resistance

Abstract : Francisella tularensis is one of the most virulent pathogenic bacteria causing the acute human respiratory disease tularemia. While the mechanisms underlying F. tularensis pathogenesis are largely unknown, previous studies have shown that a F. novicida transposon mutant with insertions in a gene coding for a putative lysine decarboxylase was attenuated in mouse spleen, suggesting a possible role of its protein product as a virulence factor. Therefore, we set out to structurally and functionally characterize the F. novicida lysine decarboxylase, which we termed LdcF. Here, we investigate the genetic environment of ldcF as well as its evolutionary relationships with other basic AAT-fold amino acid decarboxylase superfamily members, known as key actors in bacterial adaptative stress response and polyamine biosynthesis. We determine the crystal structure of LdcF and compare it with the most thoroughly studied lysine decarboxylase, E. coli LdcI. We analyze the influence of ldcF deletion on bacterial growth under different stress conditions in dedicated growth media, as well as in infected macrophages, and demonstrate its involvement in oxidative stress resistance. Finally, our mass spectrometry-based quantitative proteomic analysis enables identification of 80 proteins with expression levels significantly affected by ldcF deletion, including several DNA repair proteins potentially involved in the diminished capacity of the F. novicida mutant to deal with oxidative stress. Taken together, we uncover an important role of LdcF in F. novicida survival in host cells through participation in oxidative stress response, thereby singling out this previously uncharacterized protein as a potential drug target
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https://hal.archives-ouvertes.fr/hal-03118173
Contributeur : Céline Brochier-Armanet Connectez-vous pour contacter le contributeur
Soumis le : lundi 25 janvier 2021 - 08:49:54
Dernière modification le : vendredi 1 avril 2022 - 03:44:36
Archivage à long terme le : : lundi 26 avril 2021 - 18:32:21

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Felix et al. Nature.pdf
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Jan Felix, Claire Siebert, Juila Ducassou, Jérôme Nigou, Pierre Garcia, et al.. Structural and functional analysis of the Francisella lysine decarboxylase as a key actor in oxidative stress resistance. Scientific Reports, Nature Publishing Group, 2021, 11 (1), pp.972. ⟨10.1038/s41598-020-79611-5⟩. ⟨hal-03118173⟩

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