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Article Dans Une Revue Journal of Synchrotron Radiation Année : 2022

Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

Résumé

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive ( e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.

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Imagerie
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Origine : Publication financée par une institution

Dates et versions

hal-03839350 , version 1 (04-11-2022)

Identifiants

Citer

Simone Sala, Yuhe Zhang, Nathaly de la Rosa, Till Dreier, Maik Kahnt, et al.. Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline. Journal of Synchrotron Radiation, 2022, 29 (3), pp.807-815. ⟨10.1107/S1600577522001874⟩. ⟨hal-03839350⟩
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