Boudewijn
VAN DER SANDEN

Chercheur
Team INSYGHT
[info]
Address: Bâtiment Jean Roget, Place du Commandant Nal, Domaine de la Merci
Office: 428a

My primary research focuses on cell therapy applications, such as artificial pancreas and skin. In collaboration with chemists from the interdisciplinary physics lab (LiPhy), we photopolymerize gelatin (GelMA) matrices using digital light processing or two-photon polymerization for the macro-encapsulation of pancreatic islets and large skin wound dressings. These matrices include bio-optical sensors for monitoring cell metabolism locally before and after implantation. As the scientific director of an Intravital Microscopy Platform (GiS-IBiSA and France Life Imaging) at the medical faculty of the Grenoble Alpes University, I have easy access to two-photon and photo-acoustic microscopy (LiPhy) for optimizing cell therapy protocols both in vitro and in vivo.

One of my roles in the SyNaBi team is to analyze the stability and biocompatibility of new devices in a transgenic mouse model that expresses eGFP in mononuclear phagocytes. In this model, inflammation processes and their treatment can be tracked over time on a microscopic scale. It is crucial to prevent chronic inflammation and fibrosis formation around implants, as these conditions can hinder biochemical and cellular exchanges with host tissues.

1.           Sanden B Van Der, Gredy L, Wion D, Stephan O. Acta Biomaterialia 3D two-photon polymerization of smart cell gelatin – collagen matrixes with incorporated ruthenium complexes for the monitoring of local oxygen tensions. Acta Biomater. 2021;130:172–82.

2.           Menassol G, Sanden B Van Der, Gredy L, Arnol C, Divoux T, Martin DK, et al. Gelatine–collagen photo-crosslinkable 3D matrixes for skin regeneration. Biomaterials-science. 2024;12:1738–49.

Research themes
  1. Development and validation of new and smart gelatin (GelMA) matrixes for cell therapy applications.
  2. Inflammation imaging
  3. Intravital microscopy: two-photon and photo-acoustic

 

 

Keywords

Cell therapy, smart GelMA matrixes, optical biosensors, intravital microscopy, inflammation imaging.

artificial pancreas and skin.

 

  1. Par Raphaël Serduc  sous la direction de Chantal Rémy et de Boudewijn Van Der Sanden - Génie biologique et médical - Université Joseph Fourier (Grenoble ; 1971-2015) : Effets de la radiothérapie par microfaisceaux synchrotron sur la microvascularisation cérébrale saine et tumorale chez la souris, 2006.
  2. Par Pascale Vérant  sous la direction de Jean-Claude Vial et de Boudewijn Van Der Sanden - Physique - Université Joseph Fourier (Grenoble ; 1971-2015) Imagerie intravitale par microscopie biphotonique : application à l'étude des effets de la radiothérapie synchrotron par microfaisceaux sur la microvascularisation corticale de la souris, 2006
  3. Par Clément Ricard  sous la direction de Boudewijn Van Der Sanden et de Christoph Segebarth - Neurosciences. Neurobiologie - Université Joseph Fourier (Grenoble ; 1971-2015) Effets de la photoactivation par irradiation synchroton sur la microvascularisation et sur les tissus cérébraux chez la souris saine ou porteuse d'un gliome : développements en microscopie biphotonique et essais précliniques, 2008
  4. Par Mathieu Maurin  sous la direction de Patrice Baldeck et de Boudewijn Van Der Sanden - Physique - Grenoble, Nanoparticules fluorescentes à base de Pluronic : application à l'imagerie intravitale de la vascularisation par microscopie à deux photons et au transport de molécules, 2011
  5. Par Michel Sarraf  sous la direction de Boudewijn Van Der Sanden et de Adriana Teodora Perles-Barbacaru - Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine et l'environnement - Université Grenoble Alpes (ComUE), Evaluation non-invasive des Gliomes par Imagerie Résonance Magnétique : Effets des traitements anti-angiogéniques (Avastin) sur la microvascularisation et la microarchitecture tumorale et péritumorale,  2019
  6. Par Nikita RAJKUMARI: Photoswitchable Nanoparticles for High-Contrast Photoacoustic Microscopy to Image Inflammation in Microvasculature, Directeur de thèse: Christophe Blanquart – Boudewijn van der Sanden, en cours
Publications

A short selection of my 102 publications

B.P.J. Van Der Sanden, H.J.A. In ’t Zandt, L. Hoofd, R.A. De Graaf, K. Nicolay, P.F.J.W. Rijken, A.J. Van Der Kogel, A. Heerschap, Global HDO uptake in human glioma xenografts is related to the perfused capillary distribution, Magn. Reson. Med. 42 (1999) 479–489. doi:10.1002/(SICI)1522-2594(199909)

B.P. van der Sanden, T.H. Rozijn, P.F. Rijken, H.P. Peters, A. Heerschap, A.J. van der Kogel, W.M. Bovée, Noninvasive assessment of the functional neovasculature in 9L-glioma growing in rat brain by dynamic 1H magnetic resonance imaging of gadolinium uptake., J. Cereb. Blood Flow Metab. 20 (2000) 861–870. doi:10.1097/00004647-200005000-00013.

 J. Schepers, W.B. Veldhuis, R. Pauw, J. De Groot, M.J.P. Van Osch, K. Nicolay, B. van der Sanden, Comparison of FAIR Perfusion Kinetics With DSC-MRI and Functional Histology in a Model of Transient Ischemia, Magn. Reson. Med. 51 (2004) 312–320. doi:10.1002/mrm.10691.

U. Gimenez, A.-T. Perles-Barbacaru, A. Millet, F. Appaix, M. El-Atifi, K. Pernet-Gallay, B. van der Sanden, F. Berger, H. Lahrech, Microscopic DTI accurately identifies early glioma cell migration: correlation with multimodal imaging in a new glioma stem cell model., NMR Biomed. 29 (2016) 1553–1562. doi:10.1002/nbm.3608.

 V.-A. Maraloiu, F. Appaix, A. Broisat, D. Le Guellec, V.S. Teodorescu, C. Ghezzi, B. van der Sanden, M.-G. Blanchin, Multiscale investigation of USPIO nanoparticles in atherosclerotic plaques and their catabolism and storage in vivo., Nanomedicine. 12 (2016) 191–200. doi:10.1016/j.nano.2015.08.005.

B.P.J. Van Der Sanden, P. Rijken, A. Heerschap, H. Bernsen, A.J. Van Der Kogei, In vivo 31p magnetic resonance spectroscopy and morphometric analysis of the perfused vascular architecture of human glioma xenografts in nude mice, Br. J. Cancer. 75 (1997) 1432–1438.

 H. Dehnad, H. Kal, T. Stam, I. Gademan, R. van Moorselaar, B. van der Sanden, Response to motexafin gadolinium and ionizing radiation of experimental rat prostate and lung tumors, Int J Radiat Oncol Biol Phys. 57 (2003) 787–793. doi:10.1016/S0360-3016(03)00661-8.

J.O. Barentsz, M. Engelbrecht, G.J. Jager, J.A. Witjes, J. De LaRosette, B.P.J. Van Der Sanden, H.J. Huisman, A. Heerschap, Fast dynamic gadolinium-enhanced MR imaging of urinary bladder and prostate cancer, J. Magn. Reson. Imaging. 10 (1999) 295–304. doi:10.1002/(SICI)1522-2586(199909)10:3

 B.P.J. Van Der Sanden, A. Heerschap, L. Hoofd, A.W. Simonetti, K. Nicolay, A. Van Der Toorn, W.N.J.M. Colier, A.J. Van Der Kogel, Effect of carbogen breathing on the physiological profile of human glioma xenografts, Magn. Reson. Med. 42 (1999) 490–499. doi:10.1002/(SICI)1522-2594(199909)42.

B.P.J. Boudewijn, A. Heerschap, A.W. Simonetti, P.F.J.W. Rijken, H.P.W. Peters, G. Stb̈en, A.J. Van Der Kogel, Characterization and validation of noninvasive oxygen tension measurements in human glioma xenografts by 19F-MR relaxometry, Int. J. Radiat. Oncol. Biol. Phys. 44 (1999) 649–658. doi:10.1016/S0360-3016(98)00555-0.

R.J. Gillies, Z.M. Bhujwalla, J. Evelhoch, M. Garwood, M. Neeman, S.P. Robinson, C.H. Sotak, B. Van Der Sanden, Applications of magnetic resonance in model systems: tumor biology and physiology., Neoplasia (New York, NY). 2 (2000) 139–151. doi:10.1038/sj.neo.7900076.

S.P. Robinson, P.F.J.W. Rijken, F.A. Howe, P.M.J. McSheehy, B.P.J. Van der Sanden, A. Heerschap, M. Stubbs, A.J. Van der Kogel, J.R. Griffiths, Tumor vascular architecture and function evaluated by non-invasive susceptibility MRI methods and immunohistochemistry, J. Magn. Reson. Imaging. 17 (2003) 445–454. doi:10.1002/jmri.10274.

R. Serduc, P. Vérant, J.-C. Vial, R. Farion, L. Rocas, C. Rémy, T. Fadlallah, E. Brauer, A. Bravin, J. Laissue, H. Blattmann, B. van der Sanden, In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature, Int. J. Radiat. Oncol. 64 (2006) 1519–1527. doi:10.1016/j.ijrobp.2005.11.047.

R. Serduc, T. Christen, J. Laissue, R. Farion, A. Bouchet, B. van der Sanden, C. Segebarth, E. Brauer-Krisch, G. Le Duc, A. Bravin, C. Remy, E.L. Barbier, Brain tumor vessel response to synchrotron microbeam radiation therapy : a short-term in vivo study, Phys. Med. Biol. 53 (2008) 3609–3622. doi:10.1088/0031-9155/53/13/015.

P. Vérant, C. Ricard, R. Serduc, J.-C. Vial, B. van der Sanden, In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B, J. Biomed. Opt. 13 (2008) 1–5. doi:10.1117/1.3041163.

P. Vérant, R. Serduc, B. van der Sanden, R. Chantal, C. Ricard, J.A. Coles, J.-C. Vial, Subtraction method for intravital two-photon microscopy: intraparenchymal imaging and quantification of extravasation in mouse brain cortex, J. Biomed. Opt. 13 (2008) 011002. doi:10.1117/1.2870083.

R. Serduc, Y. van de Looij, G. Francony, O. Verdonck, B. van der Sanden, J. Laissue, R. Farion, E. Brauer-Krisch, E.A. Siegbahn, A. Bravin, Y. Prezado, C. Segebarth, C. Remy, H. Lahrech, Characterization and quantification of cerebral edema induced by synchrotron x-ray microbeam radiation therapy, Phys. Med. Biol. 53 (2008) 1153–1166. doi:10.1088/0031-9155/53/5/001.

P. Vérant, R. Serduc, B. Van Der Sanden, C. Rémy, J.-C. Vial, A Direct Method for Measuring Mouse Capillary Cortical Blood Volume Using Multiphoton Laser Scanning Microscopy, J. Cereb. Blood Flow Metab. 27 (2007) 1072–1081. doi:10.1038/sj.jcbfm.9600415.

C. Ricard, M. Fernandez, J. Gastaldo, L. Dupin, L. Somveille, R. Farion, H. Requardt, J.-C. Vial, H. Elleaume, C. Segebarth, B. van der Sanden, Short-term effects of synchrotron irradiation on vasculature and tissue in healthy mouse brain, J. Synchrotron Radiat. 16 (2009) 477–483. doi:10.1107/S0909049509015428.

B. Van Der Sanden, E. Bräuer-Krisch, E.A. Siegbahn, C. Ricard, J.C. Vial, J. Laissue, Tolerance of arteries to microplanar X-ray beams, Int. J. Radiat. Oncol. Biol. Phys. 77 (2010) 1545–1552. doi:10.1016/j.ijrobp.2010.02.019.

C. Ricard, M. Fernandez, H. Requardt, D. Wion, J.C. Vial, C. Segebarth, B. Van Der Sanden, Synergistic effect of cisplatin and synchrotron irradiation on F98 gliomas growing in nude mice, J. Synchrotron Radiat. 20 (2013) 777–784. doi:10.1107/S0909049513016567.

S.C. Boca, M. Four, A. Bonne, B. Van Der Sanden, S. Astilean, P.L. Baldeck, G. Lemercier, An ethylene-glycol decorated ruthenium ( II ) complex for two-photon photodynamic therapy w, Chem. Commun. 14 (2009) 4590–4592. doi:10.1039/b907143a.

A. Hayek, A. Grichine, T. Huault, C. Ricard, F. Bolze, B. Van Der Sanden, J.-C. Vial, Y. Mély, A. Duperray, P.L. Baldeck, J.-F. Nicoud, Cell-permeant cytoplasmic blue fluorophores optimized for in vivo two-photon microscopy with low-power excitation., Microsc. Res. Tech. 70 (2007) 880–5. doi:10.1002/jemt.20493.

M. Maurin, L. Vurth, J. Vial, P. Baldeck, S.R. Marder, B. van der Sanden, O. Stephan, Fluorescent Pluronic nanodots for in vivo two-photon imaging, Nanotechnology. 20 (2009) 235102–235107. doi:10.1088/0957-4484/20/23/235102.

T.F. Stefanello, B. Couturaud, A. Szarpak-Jankowska, D. Fournier, B. Louage, F.P. Garcia, C. V. Nakamura, B.G. De Geest, P. Woisel, B. van der Sanden, R. Auzely-velty, Coumarin-containing thermoresponsive hyaluronic acid-based nanogels as delivery systems for anticancer chemotherapy, Nanoscale. 24 (2017) 12150–12162. doi:10.1039/c7nr03964f.

S. Pascal, S. Denis-Quanquin, F. Appaix, A. Duperray, A. Grichine, B. Le Guennic, D. Jacquemin, J. Cuny, S.-H. Chi, J.W. Perry, B. van der Sanden, C. Monnereau, C. Andraud, O. Maury, Keto-polymethines: a versatile class of dyes with outstanding spectroscopic properties for in cellulo and in vivo two-photon microscopy imaging., Chem. Sci. 8 (2017) 381–394. doi:10.1039/c6sc02488b.

X. Yan, M. Remond, Z. Zheng, E. Hoibian, C. Soulage, S. Chambert, C. Andraud, B. Van der Sanden, F. Ganachaud, Y. Bretonnière, J. Bernard, General and Scalable Approach to Bright, Stable, and Functional AIE Fluorogen Colloidal Nanocrystals for in Vivo Imaging, ACS Appl. Mater. Interfaces. 10 (2018) 25154–25165. doi:10.1021/acsami.8b07859.

A. Niehl, F. Appaix, S. Boscá, B. van der Sanden, J.-F. Nicoud, F. Bolze, M. Heinlein, Fluorescent Tobacco mosaic virus-Derived Bio-Nanoparticles for Intravital Two-Photon Imaging, Front. Plant Sci. 6 (2016). doi:10.3389/fpls.2015.01244.

Z. Zheng, F. Caraguel, L. Yuan-Yuan, C. Andraud, B. van der Sanden, Y. Bretonnière, RSC Advances nanoparticles : perfect blood pool fl uorophores for biphotonic in vivo imaging of the leaky tumour, RSC Adv. 6 (2016) 94200–94205. doi:10.1039/c6ra17438h.

B. Mettra, F. Appaix, J. Olesiak-banska, T. Le Bahers, A. Leung, M. Samoc, B. Van Der Sanden, C. Monnereau, C. Andraud, A Fluorescent Polymer Probe with High Selectivity towards Vascular Endothelial Cells for and Beyond Non-Invasive Two-Photon Intravital Imaging of brain vasculature, ACS Appl. Mater. Interfaces. 13 (2016) 17047–17059. doi:10.1021/acsami.6b02936.

O. Hugon, I.A. Paun, C. Ricard, B. van der Sanden, E. Lacot, O. Jacquin, A. Witomski, Cell imaging by coherent backscattering microscopy using frequency-shifted optical feedback in a microchip laser, Ultramicroscopy. 108 (2008) 523–528. doi:10.1016/j.ultramic.2007.08.009.

V. Girardeau, O. Jacquin, O. Hugon, B. Riviere, B. van der Sanden, E. Lacot, Photo-acoustic tomography based on laser optical feedback imaging of surface displacements To cite this version : HAL Id : hal-02282968 Photo - Acoustic Tomography ( PAT ) based on Laser Optical Feedback Imaging ( LOFI ) of surface displacements, Appl. Opt. 10 (2019) 7195–7204. doi:10.1364/AO.58.007195.

B. van der Sanden, O. Hugon, M. Inglebert, O. Jacquin, E. Lacot, Vascular bifurcation mapping with photoacoustic microscopy, Biomed. Opt. Express. 11 (2020) 1298–1305. doi:10.1364/boe.383583.

O. Hugon, B. van der Sanden, M. Inglebert, O. Jacquin, C. Misbah, E. Lacot, Multi-wavelength photo-acoustic microscopy in the frequency domain for simultaneous excitation and detection of dyes, Biomed. Opt. Express. 10 (2019) 932–943. doi:10.1364/BOE.10.000932.

D. Tarus, L. Hamard, F. Caraguel, D. Wion, A. Szarpak-Jankowska, B. van der Sanden, R. Auzely-velty, Design of hyaluronic acid hydrogels to promote neurite outgrowth in three dimensions, ACS Appl. Mater. Interfaces. 28 (2016) 25051–25059. doi:10.1021/acsami.6b06446.

F. Caraguel, A.C. Lesart, F. Estève, B. Van Der Sanden, A. Stéphanou, Towards the design of a patient-specific virtual tumour, Comput. Math. Methods Med. 2016 (2016) 1–12. doi:10.1155/2016/7851789.

L. Sancey, S. Kotb, C. Truillet, F. Appaix, A. Marais, E. Thomas, B. van der Sanden, J.-P. Klein, B. Laurent, M. Cottier, R. Antoine, P. Dugourd, G. Panczer, F. Lux, P. Perriat, V. Motto-Ros, O. Tillement, Long-Term in Vivo Clearance of Gadolinium-Based AGuIX Nanoparticles and Their Biocompatibility after Systemic Injection, ACS Nano. 9 (2015) 2477–2488. doi:10.1021/acsnano.5b00552.

T. Fernandes, A. Szarpak-jankowska, F. Appaix, B. Louage, L. Hamard, B.G. De Geest, B. Van Der Sanden, C. Vataru, R. Auzély-velty, Acta Biomaterialia Thermoresponsive hyaluronic acid nanogels as hydrophobic drug carrier to macrophages, Acta Biomater. 10 (2014) 4750–4758. doi:10.1016/j.actbio.2014.07.033.

B. Van Der Sanden, L. Gredy, D. Wion, O. Stephan, Acta Biomaterialia 3D two-photon polymerization of smart cell gelatin – collagen matrixes with incorporated ruthenium complexes for the monitoring of local oxygen tensions, Acta Biomater. 130 (2021) 172–182. doi:10.1016/j.actbio.2021.06.021.

G. Menassol, B. Van Der Sanden, L. Gredy, C. Arnol, T. Divoux, D.K. Martin, O. Stephan, Gelatine–collagen photo-crosslinkable 3D matrixes for skin regeneration, Biomaterials-Science. 12 (2024) 1738–1749. doi:10.1039/d3bm01849k.

F. Appaix, S. Girod, S. Boisseau, J. Römer, J.C. Vial, M. Albrieux, M. Maurin, A. Depaulis, I. Guillemain, B. van der Sanden, Specific in vivo staining of astrocytes in the whole brain after intravenous injection of sulforhodamine dyes, PLoS One. 7 (2012) 1–13. doi:10.1371/journal.pone.0035169.

M. Maurin, O. Stéphan, J.-C. Vial, S.R. Marder, B. van der Sanden, Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles., J. Biomed. Opt. 16 (2011) 036001. doi:10.1117/1.3548879.

D. Wion, F. Appaix, M. Burruss, F. Berger, B. van der Sanden, Cancer research in need of a scientific revolution : Using ‘ paradigm shift ’ as a method of investigation, J. Biosci. (2015). doi:10.1007/s12038-015-9543-3.

F. Appaix, M.-F. Nissou, B. van der Sanden, M. Dreyfus, F. Berger, J.-P. Issartel, D. Wion, Brain mesenchymal stem cells: The other stem cells of the brain?, World J. Stem Cells. 6 (2014) 134–43. doi:10.4252/wjsc.v6.i2.134.

M.-F. Nissou, M. El Atifi, A. Guttin, C. Godfraind, C. Salon, E. Garcion, B. van der Sanden, J.-P. Issartel, F. Berger, D. Wion, Hypoxia-induced expression of VE-cadherin and filamin B in glioma cell cultures and pseudopalisade structures., J. Neurooncol. 113 (2013) 239–49. doi:10.1007/s11060-013-1124-4.

D. Ratel, B. van der Sanden, D. Wion, Glioma resection and tumor recurrence: back to Semmelweis, Neuro. Oncol. 18 (2016) 1688–1689. doi:10.1093/neuonc/now201.

B. van der Sanden, D. Ratel, F. Berger, D. Wion, Glioma Recurrence following Surgery: Peritumoral or Perilesional?, Front. Neurol. 7 (2016) 1–2. doi:10.3389/fneur.2016.00052.

L. Hamard, D. Ratel, L. Selek, F. Berger, B. van der Sanden, D. Wion, The brain tissue response to surgical injury and its possible contribution to glioma recurrence, J. Neurooncol. 128 (2016) 1–8. doi:10.1007/s11060-016-2096-y.

M. Sarraf, A.T. Perles-Barbacaru, M.F. Nissou, B. Van Der Sanden, F. Berger, H. Lahrech, Rapid-Steady-State-T1 signal modeling during contrast agent extravasation: Toward tumor blood volume quantification without requiring the arterial input function, Magn. Reson. Med. 73 (2015) 1005–1014. doi:10.1002/mrm.25218.

F. Caraguel, A.C. Lesart, F. Estève, B. Van Der Sanden, A. Stéphanou, Towards the design of a patient-specific virtual tumour, Comput. Math. Methods Med. 2016 (2016). doi:10.1155/2016/7851789.

A. Lesart, B. Van Der Sanden, L. Hamard, On the importance of the submicrovascular network in a computational model of tumour growth, Microvasc. Res. 84 (2012) 188–204. doi:10.1016/j.mvr.2012.06.001.

A.T. Perles-Barbacaru, B.P.J. van der Sanden, R. Farion, H. Lahrech, How stereological analysis of vascular morphology can quantify the blood volume fraction as a marker for tumor vasculature: comparison with magnetic resonance imaging., J. Cereb. Blood Flow Metab. 32 (2012) 489–501. doi:10.1038/jcbfm.2011.151.

 

Teachings

Plenary lectures (5h/year) on Small Animal Imaging at the Faculty of Biology and Pharmacology of Grenoble Alpes University

Projects
  1. ANR IMAGin 2023 - 2027 : Engineering of All-Organic Fluorescent Nanoparticles for Optical Imaging
  2. ANR AZOTICS 2022 - 2026 : Photoswitchable NPs for High-Contrast Photoacoustics Microscopy to Image Inflammation in Microvasculature
  3. Prematuration CNRS: BEPI, 2022-2023, Un Pancréas artificiel implantable pour une thérapie cellulaire du diabète
  4. Maturation SATT Linksium: BioPi 2023-2024,  Bioréacteur pancréatique implantable
  5. IDEX 2024 - 2025: Softcages, Cell sensitivity to stiffness in 3D geometries with independently controlled micron-scaled stiffness and cell-scaled porosity.
  6. France Life Imaging 2023 - 2024: IMPACTION, Nouvelles sondes pour l'Imagerie Photoacoustique de l'inflammation.
Partnerships

Dr. Olivier Stephan, Laetitia Gredy, Move team, Interdiscplinary physics labs (LiPhy), Grenoble.

Dr. Olivier Hugon, Prof. Eric Lacot, OPTIMA team, Interdiscplinary physics labs (LiPhy), Grenoble.

Dr. Yann Bretonnière, Lab. de Chimie, ENS de Lyon.

Dr. Julien Bernard, INSA, Lyon.

Prof Simona Mura, Univ. Paris Sud.

Prof. Elena Ishow, CEISAM, Nantes.

Dr. Christophe Blanquart, CRCINA, Nantes.

Dr. Frederic Bolze, CAMB, Unistra, Strasbourg.