Nitrile rubber is well recognized for its strength and oil resistance characteristic. The resistance to swelling in hydrocarbon oils permits it to be widely utilized in the industrial and automotive field, such as seals, gaskets, rollers and packers. However, petroleum-based fuels are no longer dependable source of energy in terms of long term availability, increasing cost and environmental preservation. These undeniable issues have made biofuel a more attractive alternative energy source since it is known to have better sustainability and economic feasibility if compared to other choices of renewable energy sources. Despite the many advantages of biofuel, the material compatibility, in particularly rubbers, in biofuel systems remains as a challenge till today.