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Physicochemical Evidence that Francisella FupA and FupB Proteins Are Porins

Claire Siebert 1 Corinne Mercier 2 Donald Martin 3 Patricia Renesto 1 Beatrice Schaack 1, 4
1 TIMC-TheREx - Thérapeutique Recombinante Expérimentale
TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
2 TIMC-GEM - Génomique et Évolution des Microorganismes
TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
3 TIMC-SyNaBi - Systèmes Nanobiotechnologiques et Biomimétiques
TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
Abstract : Responsible for tularemia, Francisella tularensis bacteria are highly infectious Gram-negative, category A bioterrorism agents. The molecular mechanisms for their virulence and resistance to antibiotics remain largely unknown. FupA (Fer Utilization Protein), a protein mediating high-affinity transport of ferrous iron across the outer membrane, is associated with both. Recent studies demonstrated that fupA deletion contributed to lower F. tularensis susceptibility towards fluoroquinolones, by increasing the production of outer membrane vesicles. Although the paralogous FupB protein lacks such activity, iron transport capacity and a role in membrane stability were reported for the FupA/B chimera, a protein found in some F. tularensis strains, including the live vaccine strain (LVS). To investigate the mode of action of these proteins, we purified recombinant FupA, FupB and FupA/B proteins expressed in Escherichia coli and incorporated them into mixed lipid bilayers. We examined the porin-forming activity of the FupA/B proteoliposomes using a fluorescent 8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt (ANTS) probe. Using electrophysiology on tethered bilayer lipid membranes, we confirmed that the FupA/B fusion protein exhibits pore-forming activity with large ionic conductance, a property shared with both FupA and FupB. This demonstration opens up new avenues for identifying functional genes, and novel therapeutic strategies against F. tularensis infections.
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https://hal.archives-ouvertes.fr/hal-03018927
Contributeur : Patricia Renesto <>
Soumis le : lundi 23 novembre 2020 - 10:29:47
Dernière modification le : mardi 8 décembre 2020 - 03:10:57
Archivage à long terme le : : mercredi 24 février 2021 - 18:42:25

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2020-Siebert_ijms-21-05496.pdf
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Claire Siebert, Corinne Mercier, Donald Martin, Patricia Renesto, Beatrice Schaack. Physicochemical Evidence that Francisella FupA and FupB Proteins Are Porins. International Journal of Molecular Sciences, MDPI, 2020, ⟨10.3390/ijms21155496⟩. ⟨hal-03018927⟩

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