Amoebae can promote the survival of Francisella species in the aquatic environment - Archive ouverte HAL Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Amoebae can promote the survival of Francisella species in the aquatic environment

Aurélie Hennebique 1, 2 Julien Peyroux Camille Brunet Amandine Martin Thomas Henry Masa Knezevic Marina Santic Sandrine Boisset Max Maurin
2 TIMC-TrEE - Translational microbial Evolution and Engineering
TIMC - Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525
Abstract : Francisella tularensis, a tier select agent, is the causative bacterium of tularemia, a zoonosis with a large animal reservoir. However, F. tularensis, like many other Francisella species (including F. novicida and F. philomiragia), is assumed to have an aquatic reservoir. The mechanisms of Francisella species persistence in surface water remain poorly characterized. In this study, we deeply investigated the long-term interactions of the tularemia agent F. tularensis subsp. holarctica, F. novicida or F. philomiragia with amoebae of the Acanthamoeba species. In amoeba plate screening tests, all the Francisella species tested resisted the attack by amoebae. In in vitro infection models, intra-amoebic growth of Francisella varied according to the involved bacterial species and strains, but also the amoeba culture medium used. In co-culture models, the amoebae favoured Francisella survival over 16 days. Such enhanced survival was likely dependent on direct contact between bacteria and amoebae for F. novicida and on amoeba-excreted compounds for F. novicida and for F. tularensis. In a spring water co-culture model, amoebae again enhanced F. novicida survival and preserved bacterial morphology. Overall, our results demonstrate that amoebae likely promote Francisella survival in aquatic environments, including the tularemia agent F. tularensis. However, bacteria-amoebae interactions are complex and depend on the Francisella species considered.
Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Aurélie Hennebique Connectez-vous pour contacter le contributeur
Soumis le : vendredi 5 février 2021 - 12:08:28
Dernière modification le : mardi 19 octobre 2021 - 11:06:02
Archivage à long terme le : : vendredi 7 mai 2021 - 08:26:50


Francisella and Amoebae_Revise...
Fichiers produits par l'(les) auteur(s)




Aurélie Hennebique, Julien Peyroux, Camille Brunet, Amandine Martin, Thomas Henry, et al.. Amoebae can promote the survival of Francisella species in the aquatic environment. Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2021, ⟨10.1080/22221751.2021.1885999⟩. ⟨hal-03132662⟩



Consultations de la notice


Téléchargements de fichiers