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Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA

Chantal Dumestre-Pérard 1 Nicole Thielens 2
1 TIMC-IMAG-BNI - Barrière Naturelle et Infectiosité
TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications Grenoble - UMR 5525
Abstract : Ficolins are recognition proteins of the lectin pathway of the complement system and also play an important role in innate immunity and in the maintenance of tissue homeostasis. They deserve special attention in the context of autoimmunity since they are involved in the uptake of dying cells. Because the monitoring of systemic lupus erythematosus (SLE) patients is particularly difficult, it is crucial to find new relevant serum biomarkers. The ability to detect autoantibodies in the patients' sera provides a diagnostic and prognostic advantage. We describe in this chapter quantitative enzyme linked immunosorbent assays (ELISA) to detect the presence of autoantibodies targeting ficolin-2 and ficolin-3 in human sera. Recombinant ficolins produced in a mammalian expression system are used as coating antigens. The described in-house ELISAs provide a valuable tool to efficiently quantify anti-ficolin autoantibodies in the sera of SLE patients.
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Soumis le : samedi 17 avril 2021 - 10:08:37
Dernière modification le : mardi 19 octobre 2021 - 11:20:59

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Chantal Dumestre-Pérard, Nicole Thielens. Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA. Methods in Molecular Biology, Humana Press/Springer Imprint, 2021, 2227, pp.121-132. ⟨10.1007/978-1-0716-1016-9_12⟩. ⟨hal-03200976⟩

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