Electrochemical biosensing of glucose based on the enzymatic reduction of glucose - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Sensors Année : 2022

Electrochemical biosensing of glucose based on the enzymatic reduction of glucose

Résumé

In this work, the enzyme aldehyde reductase, also known as aldose reductase, was synthesized and cloned from a human gene. Spectrophotometric measurements show that in presence of the nicotinamide adenine dinucleotide phosphate cofactor (NADPH), the aldehyde reductase catalyzed the reduction of glucose to sorbitol. Electrochemical measurements performed on an electrodeposited poly(methylene green)-modified gold electrode showed that in the presence of the enzyme aldehyde reductase, the electrocatalytic oxidation current of NADPH decreased drastically after the addition of glucose. These results demonstrate that aldehyde reductase is an enzyme that allows the construction of an efficient electrochemical glucose biosensor based on glucose reduction.
Fichier principal
Vignette du fichier
sensors-22-07105.pdf (2 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03798340 , version 1 (07-10-2022)

Licence

Identifiants

Citer

Thomas Soranzo, Awatef Ben Tahar, Ayman Chmayssem, Marc Zelsmann, Pankaj Vadgama, et al.. Electrochemical biosensing of glucose based on the enzymatic reduction of glucose. Sensors, 2022, 22 (19), pp.7105. ⟨10.3390/s22197105⟩. ⟨hal-03798340⟩
1216 Consultations
48 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More